, 1997). , 2021). Next,. After correcting for negative controls and quench standards, the soil enzyme activities were expressed in units of nmol h −1 g −1 soil (German et al. Microplate readers, multi-mode, SpectraMax® i3x. TP medium was used as a blank and its fluorescence intensity was subtracted from that of mCherry or from chlorophyll auto-fluorescence intensity. In dual reporter gene assays, cells are transfected with two vectors, the first containing an experimental reporter gene coupled to a regulated promoter of interest, andSpectraMax i3x The SpectraMax i3x from Molecular Devices is a multi-mode detection system that evolves with your future needs and offers an unlimited breadth of application possibilities. Quantification of NETs was performed with the SpectraMax i3x microplate-reader (Molecular Devices, United Kingdom). Dual auto injector cartridges for the SpectraMax® i3x Multi-Mode Microplate Detection Platform allows you to expand your research capabilities to include flash based applications, including dual luciferase and ATP assays. Bioz Stars score: 98/100, based on 1 PubMed citations. 3: The cuvette chamber. HTRF IP standard curve. It also integrates with the. SpectraMax M2, M2e; SpectraMax M3, M4, M5, M5e; FilterMax™ Multi-Mode Detection. Wokingham, Berkshire RG41 5TS United Kingdom. Contact Us Phone: +1. The microplate was then placed in a SpectraMax i3x reader and incubated at 37°C for the duration of the kinetic measurement. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,Danaher Inc spectramax i3x Spectramax I3x, supplied by Danaher Inc, used in various techniques. The PMT gain can be set to manual, high, medium, low, or automatic (Figure 3A). 1. The SpectraMax ® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular. 3: The cuvette chamber. 1. StainFree Cell Detection Algorithm. 3. Easily upgrade or switch between detection modes with user-exchangeable modules – just open the drawer and insert the module you want. This way you’ll save time on finding the necessary info. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. Patented user-exchangeable cartridge design expands the system's detection capability making it. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. For each assay kit, a preconfigured protocol is included in SoftMax Pro Software to facilitate the acquisition and analysis of data for a simplified workflow. 2C). Ease of use. If desired, a high-range standard curve may be prepared from 1 ng/mL to 1 μg/mL, or a low-range standard curve may be prepared from 25 pg/mL to 25 ng/mL. The SpectraMax® i3x multi-mode microplate reader is a configurable, versatile microplate reader that evolves with your future needs and allows you to investigate every aspect of a cellular pathway. Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader This page contains the table of contents of resources available for the SpectraMax Microplate Reader Suite of Products. The heating element design of the category 1 microplate readers, such as the SpectraMax i3x Multi-Mode Microplate Reader, applies a dual area sensor for temperature control. 02, 0. Red and green fluorescence was then measured (emission at 590 and 530 nm, respectively, with excitation at 490 nm) using a fluorescence microplate reader (Spectra Max i3x, Molecular Devices Inc. User-installable and removable detection cartridges allow the detector to be configured for specific applications and easily. CRITICAL STEP Avoid air bubbles; reverse pipetting the luciferase reagent into the plate is a good way to achieve this. ) deep, preferably with access on all sides. Increased fluorescence performance. SoftMax Pro GxP software is a special release that includ es full user and permission control and audit trail generation in addition to all the dataBased on the SpectraMax i3x system, ACROBiosystems provides biomedical research and development services for customers seeking quantification and detection of antibodies-antigen interaction. In this application note, we describe how the SpectraMax ® i3x Multi-Mode Microplate Reader with SpectraMax Injector Module is used to perform calcium assays on two different cell lines. Manufacturers try to design their instruments so they can be. com Check our website for a current listing of worldwide distributors. 306 plate air cal. The SpectraMax i3x reader allows you to unravel the mysteries of science by exploring SpectraMax i3 Install Guide; SpectraMax i3; SpectraMax i3x; SpectraMax iD3; SpectraMax iD5; SpectraMax Mini; SpectraMax Mini Install Guide; SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. TNF-alpha quantification down to low picomolar levels on the SpectraMax Paradigm Platform. Additionally, user-installable options allow the SpectraMax i3 System to grow with your changing application needs to fulfill and go beyond the standard reader applications, protecting your intial investment. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. The SoftMax Pro Software provides integrated instrument control. Time Resolved Fluorescence: Solid white plates for top reads and white plates with clear bottoms for bottom reads. A SpectraMax® i3x was used to determine fluorescence with an excitation wavelength of λ = 360 nm and emission at λ = 460 nm. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Real-time measurement of extracellular oxygen depletion and acidification in cultured cells and microorganisms. 3: The cuvette chamber. **On the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. 6% for the on-board optics (monochromators). com. Benefits • User-upgradeable application modules including cellular imaging • Sensitivity across spectrum with Spectral Fusion™ Illumination • Expanded dynamic range with cooled PMT • Control and analytics provided by SoftMax® Pro Software SpectraMax i3x The SpectraMax i3x from Molecular Devices is a multi-mode detection system that evolves. SpectraMax i3x, onboard luminescence 121. As per Volza’s India Import data, Spectramax import shipments in India stood at 365, imported by 145 India Importers from 28 Suppliers. SpectraMax i3x 多功能微孔板读板机 SpectraMax Paradigm 多功能微孔板读板机 SpectraMax M5e 多功能微孔板读板机 扫一扫关注我 们的官方微信 Molecular Devices 大中华区 全国咨询服务热线: 400-820-3586 Email: info. SpectraDrop Abs Protein Quant (i3-i3x)Molecular Devices SpectraMax i3x Multi-Mode Detection Platform. No Stain Analysis: Eliminate cell staining for cell counting and confluence measurements using. Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. 4. Danaher Inc spectramax i3x. SpectraMax M5. 2. 312 gain calibration failed. The SpectraMax i2X is used for cell migration, cell. The user guide also covers the features and specifications of the SpectraMax MiniMax 300. Concentrating the excitation light on the sample through label specific optics, high levels of sensitivity and performance are reached. Learn about the SpectraMax i3x Multi-Mode Plate Reader capabilities and options in this short video. The UV-Vis spectroscopy was measured on a TU-1900Beijing PuXi instrument. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. The treatments were. 315 can't find zero order. Figure 2-23: Bottle holder To install the bottle holder: 1. 0112-0115J 9 Chapter1:Introduction TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-ModeSpectraMax MiniMax 300 イメージングサイトメーター は、研究室でのアップグレードが可能なSpectraMax i3xシステム専用のオプションで、ユーザーは複雑なイメージングシステムに投資することなく、細胞増殖を解析できます。SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. Time-resolved fluorescence was measured on the SpectraMax Multi-Mode Microplate Readers (see Table 2 for instrument settings). The NET-specificity of the detected signal was determined by incubation with DNase I (50 U/mL, Qiagen, Germany). Description 1. Unlock your i3/i3x instrument after shipment. The SpectraMax® Paradigm® Multi-Mode Microplate Reader measures absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence assays for up to 1536-well plates. Ideal for a wide range of applications, including Western Blot analysis, this SpectraMax i3x includes the additional MiniMax 3000 Imaging Cytometer module for advanced cell imaging. 0. The reader can be equipped with a NanoBRET detection for a 4-fold improvement in limit of. Spectral Fusion Illumination is a powerful combination of Xenon flash lamp and LEDs that provides unmatched signal strength and superior sensitivity across the full. Average 98 stars, based on 1 article reviews. Art-science interactive light show/animation. Whether you are looking for an intuitive, easy-to-use reader for your busy, multi-user lab, or an upgradeable system that can adapt to support your lab’s new projects and changing objectives, the i Series has you covered. Enzyme activity was presented as absolute activity in a unit of nmol g −1 dry soil h −1. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. The SpectraMax® Quant™ dsDNA Assay Kits are designed for fluorescence-based dsDNA quantitation across a broad range of concentrations. 2) Add in your protocol in Method Editor the Fluorescence Polarization strip. 314 low lamp level warning. The dynamic range of the assay is about 3. 06, 0. Every SpectraMax ® Paradigm Detection Cartridge starts with an application optimized excitation energy source either in the form of a long life, high powered LED, xenon flash lamp, or high powered laser. Examples: fiPress carefully to engage,fl fiDo not press down. Patented user-exchangeable cartridge design expands the system's detection capability making it. HTRF is a proprietary time-resolved fluoresce nce technology that overcomesThe SpectraMax i3x reader features a cooled photomultiplier tube (PMT) for low background noise in luminescence. 22 μ m fi ltration unit (Sigma, cat. Next, an AlphaLISA assay was developed to detect levels of pro-inflammatory factors produced by endothelial cells in response to TNF-alpha stimulation. The SpectraMax® i3 Platform from Molecular Devices® is a multi-mode detection system that evolves with your future needs and offers an unlimited breadth of application possibilities. 0´ 104 1. Table 3 Optimal instrument settings for Valita Titer Plus assay Fluorescence Polarization measurement on Molecular Devices microplate readers. Plate chamber design of the SpectraMax category 1 microplate readers. 1 1 10 100 e e e e e e eThe SpectraMax MiniMax 300 Imaging Cytometer option can be added at any time to the SpectraMax i3x System, providing an expandable platform that evolves with your changing needs. 312 gain calibration failed. It allows you to explore cellular . 10, and 0. AlphaScreen and AlphaLisa detection using a 680 nm excitation laser diode and a 570/100 nm emission filter. The MPO assay was conducted according to the. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Find regional support. 2 (Molecular Devices, San Jose, CA). 314 low lamp level warning. Features. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. ) wide and 65 cm (25. no. Prepare your i3/i3x instrument for shipment. Contact our support team with your questions regarding Molecular Devices products and applications. Description 1. SoftMax Pro Software enables users to quickly set up analysis of cell counts via a simple point and click user interface. flValitaTiter (i3x i3) ValitaTiter (iD5) ValitaTiter (M5 M5e Flex3) ValitaTiter (Paradigm) ValitaTiter (FilterMax F5) Assay-Specific. Figure 1. Italic and boldface type are used for emphasis. AlphaScreen and AlphaLisa detection using a 680 nm excitation laser diode and a 570/100 nm emission filter. Explore a wealth of applications with the future-ready SpectraMax i3x. The SpectraMax i3x measures monochromator-based absorbance, fluorescence and luminescence with a future-ready platform that can evolve with the researcher’s needs. 12 mg/mL) of polysaccharides were prepared after addition of glucosum anhydricum for calibration. Cet appareil ISM est confomre à la norme NMB-001 du Canada. The SpectraMax i3x Multi-Mode Detection Platform User Guide provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax i3x, a versatile and customizable microplate reader that can perform various assays and imaging tasks. Get a front row view of your cells. 99. 316 grating motor driver faulty. ) deep, preferably with access on all sides. The SpectraMax i3x reader provides theWith these results, as well as CV values below 6% for all standards, both the SpectraMax i3x and the SpectraMax iD5 readers demonstrate high sensitivity and a wide dynamic range for the IP-One assay. com The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. 5 µL anti-cAMP-Cryptate. Fluorescence at 365 nm excitation and 450 nm emission filters was determined based on a microplate fluorometer (SpectraMax i3x, Molecular Devices, Beckman Coulter, CA, USA). Spectramax I3x, supplied by Danaher Inc, used in various techniques. The SoftMax Pro Software provides integrated instrument control. In this case, the SpectraMax i3x reader works as a flash-lamp based microplate reader as described in the supporting application note “The AutoPMT™ feature for an extended dynamic range in the SpectraMax iD and M series Multi-Mode Readers”. The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. Detection cartridge modularity allows for easy addition of new read modes within minutes without. The treatments were. Prime function 20 amol. 0´ 104 8. This application note describes the use of the SPECTRAmax ® GEMINI XS from Molecular Devices to study the performance of the LIVE/DEAD ® Viability/Cytoxicity Assay Kit for animal cells. INTRODUCTION The SpectraMax M2 and SpectraMax M2e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as wellThe absorbance was recorded on a spectra Max i3x microplate reader (Molecular Devices, USA) at 450 nm. Features. , Fisher Scienti fi c, cat. Eliminate time-dependent substrate addition steps. ATP (“flash” luminescence using . XPS spectra were obtained from PHI Versaprobe II. applications, including dual luciferase and ATP assays. 3, were mixed in a final volume of 100 μL in 10 mM sodium phosphate buffer, pH 7. each new lot of plates, to ensure that the center of each well is read. Features technology from the SpectraMax i3x and MiniMax 300 Imaging Cytometer. 3) At the bottom of the strip, click on "Show. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence. 6% for the on-board optics (monochromators). Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. The Molecular Devices SpectraMax i3x Microplate Reader is a multi-mode microplate reader, able to measure UV/vis, fluorescence and luminescence. Price from $9. We would like to show you a description here but the site won’t allow us. Bound crystal violet was dissolved with 10% acetic acid and the optical density (OD) at 570 nm was determined using a Spectra Max i3X spectrophotometer (Molecular Devices). The SpectraMax® i3x multi-mode microplate reader is a configurable, versatile microplate reader that evolves with your future needs and allows you to investigate every aspect of a cellular pathway. Patented user-exchangeable cartridge design expands the system's detection capability making it. The SpectraMax® 190 spectrophotometer is ideal for most life science applications, especially DNA analysis. SpectraMax i3x Multi-Mode Detection Platform. Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. Kastelic,2 and Jian Gao 1 1Department of Clinical Veterinary Medicine, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China. Turbidity measurements were performed with a Spectra Max® i3x microplate reader to study the effect of oxidation on the kinetics of sFN formation. SpectraMax® i3x Multi-Mode Detection Platform, Molecular Devices. For aqueous solutions, our patented* PathCheck® sensor technology senses the depth of the liquid in each microplate well, and normalizes the absorbance value to a 1-cm pathlength. They were allowed to attach and grow overnight in a 37°C, 5% CO 2. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Molecular Devices SpectraMax ® i3x MultiMode Microplate Reader, in combination with luminescent assays for cell viability and cytotoxicity, provides a sensitive and rapid way to measure the number of viable cells in culture and quantify the cytotoxic effects of experimental treatment. Biocompare is the leading resource for up-to-date product information, product reviews, and new technologies for life scientists. SpectraMaxi3Multi. the SpectraMax iD3 is a fantastic plate reader for a wide range of assays. It can be upgraded with modules such as imaging cytometer, western blot imager, and injectors to expand its capabilities. 흡광 (Absorbance) 리더기. The SpectraMax® i3x Multi-Mode Detection Platform measures spectral-based absorbance, fluorescence, and luminescence with the added. The TRF intensities measured are proportional to the level of extracellular acidification in each sample. Keywords: spectramax m2 systems, spectramax m2e systems, spectramax m2/m2e readers, pathcheck sensor, spectratest abs1 absorbance, stakmax stacker Created Date:. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. The protocols are also available on the SoftMax Pro Software protocol sharing. The dual auto injector module. Description 1. Bioz Stars score: 98/100, based on 1 PubMed citations. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. Downloads. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader. 2 x 10-14. D 1 1. Example: [Drawer]. spectramax i3x (Danaher Inc)The fluorescence emission intensity spectra were recorded using a microplate reader (Spectra Max® i3x, Molecular Devices, LLC. Fluorescent Polarization: Solid black plates, as above. Multiple automated tests are provided including: • Linearity • Precision • Accuracy • Dynamic range • Alignment • Signal to noise • Signal to background • Filter checks. Fluorescence emission spectra were collected on an ELISA molecular system (Spectra Max i3x). Bookings are free, but you will need to use iLab to book. Thetypeof luciferase(Renilla. Explore a wealth of applications with the future-ready SpectraMax i3x. i3. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. SpectraMax® i3x Multi-Mode Microplate Reader with MiniMax™ imaging cytometer Integrated multi-mode microplate reader and imaging system • Ideal for cell counting, label-free imaging, and cell proliferation • Western blot, imaging, and injectors on one configurable microplate reader • Stain free, automated cell countingusing the SpectraMax DuoLuc Reporter Assay Kit on the SpectraMax i3x microplate reader. 1) Select the correct plate and the wells that you want to measure. 2. The SpectraMax® i Series Multi-Mode Mode Microplate Readers offer the ideal solution for any application or budget. Find regional support. Devices instruments including: SpectraMax®, VersaMax, VMax®, EMax®, Gemini, ThermoMax, and FlexStation® readers. Download PDF. Modularity does provide some measure of forward vision into upcoming or future experimental needs. Simplified workflow: Add cellular imaging assays in a small footprint. Time-. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. • SpectraMax i3x Injector Cartridge with SmartInject™ Technology • Expand your research capabilities to include flash-based . Be Future Ready with the SpectraMax i3x Multi-Mode Detection Platform. 99（图一）。The SpectraMax Injector System with SmartInject™ Technology features low dead volume (10 pL), overflow protection, and ensures. 313 reference gain check fail. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. In addition, Image J was used to quantify the. Bioz Stars score: 98/100, based on 1 PubMed citations. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. The SpectraMax MiniMax 300 Imaging Cytometer option can be added at any time to the SpectraMax i3x System, providing an expandable platform that evolves with your changing needs. Spectranet customer portalWatch this video to get an introduction to the Injector-Ready SpectraMax® i3x Multi-Mode Microplate Reader System. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader. Easily upgrade or switch between detection modes with user-exchangeable modules – just open the drawer and insert the module you want. Prismatic colour and motion for everyone. 2 only) SpectraMax M-series Microplate readers. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a. 315 can't find zero order. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user-configurable. Description 1. Capture flash assays with ease The SpectraMax i3x Injector Cartridge with SmartInject® Technology expands your research capabilities to include flash-based applications including dual luciferase and ATP assays. . The Molecular Devices SpectraMax i3x is a multi-mode microplate reader, able to measure UV/vis, fluorescence and luminescence. Data from Analyst®, and FLIPR® systems can also be easily imported and analyzed. 2 GxP Software is the latest, most secure software to achieve full FDA 21 CFR Part 11 and EudraLex Annex 11 compliance with. User-upgradeable applications The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. It allows you to explore cellular pathways and protein activation and expression in one system. SpectraMax i3x Multi-Mode Microplate Reader measures absorbance, fluorescence and luminescence. Reduce background noise The cooled PMT reduces background noise allowing for a more sensitive, wide dynamic range in extremely low light. 3) At the bottom of the strip, click on "Show. Description 1. The SpectraMax i3x from Molecular Devices is a multi-mode plate reader that can measure spectral-based absorbence and luminescence. Bioz Stars score: 98/100, based on 1 PubMed citations. Microplate and read height should be optimized for each new lot of microplates or assay volume used (use the Show Pre-Read Optimization options in the instrument settings in SoftMax Pro Software). Our patent-pending StainFree Cell Detection Algorithm enables cell counting and confluency measurements. Cell viability was calculated as average percent of the OD reading relative to that of control group, which was normalized to 100%. Prepare your i3/i3x instrument for shipment. Find regional support. The SpectraMax i3x reader grows with you as you continue to advance your research, enabling you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one system. User-upgradeable detection modules allow you to expand the capabilities. For aqueous solutions, our patented* PathCheck® sensor technology senses the depth of the liquid in each microplate well, and normalizes the absorbance value to a 1-cm pathlength. SpectraMax i3x Multi-Mode Microplate Reader SystemSpectraMax® i3x dari Molecular Devices adalah sistem deteksi berbasis multi-mode yang berevolusi menyesuaik. 58% for the detection module and 2. Relative mitochondrial polarization was expressed as the ratio of emission at 590 nm to that at 530 nm (Shin et al. SpectraMax® i3xMulti-ModeMicroplateReader SpectraMax® i3Multi-ModeMicroplateReader FlexStation® 3Multi-ModeMicroplateReader SpectraMax® M5eMulti-ModeMicroplateReader SpectraMax®M5Multi-ModeMicroplateReader SpectraMax® M4Multi-ModeMicroplateReader SpectraMax® M3Multi. SpectraMax i3x and Paradigm readers require the FP-FLUO. Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. ; India imports most of its Spectramax from United States, China and Austria and is the largest importer of Spectramax in the World. The corrected absorbance is within 5% of the. BRET2canbeusedtostudyvariouskindsofprotein-proteininteractions. Molecular Devices are known for both the reliability and the wide uses of their products. HTRF-compatible SpectraMax Multi-Mode Readers. 6 in. Microplate assays were performed on a multimode microplate detector (Molecular Devices Spectra Max i3x). CHO M1WT3 cells are Chinese hamster ovary cells stably transfected with the M1 muscarinic G q -coupled receptor. Accordingly, we calculated the Laurdan generalized polarization (GP) using the formula GP = (I 435 − I 490)/(I 435 +. SpectraMax i3x. Methods. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. Video: SpectraMax i3x Overview; Brochure: SpectraMax i3x Multi-Mode Detection PlatformNORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. Fluorescence Microplate Reader Comparison Chart. 4. Spectranet customer portalPage 38 SpectraMax iD3 Multi-Mode Microplate Reader User Guide Bottle Holder The bottle holder is mounted on two knobs for ease of installation, cleaning, and replacement. ; Page 2 Molecular Devices equipment, software, reagents, and consumables. Table 1. The SpectraMax i3x reader allows you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one system. Also refer to this Multimode Reader Comparison Chart. This is not due to a temperature-dependent alteration in cellular autofluorescence,. 0200-7029 Wavelength range (LUM): Visible to 650 nm Dead volume: < 10 µL with Reverse . Fluorescence intensity was measured using a Spectramax i3x plate reader (Molecular Devices) with excitation at 575 nm (bandwidth 9 nm) and emission at 608 nm (bandwidth 15 nm). The cell imaging was captured on Zeiss LSM 980 confocal microscopy. HeLa cells were plated at 15,000 cells per well in 100 µL of medium in a 96-well black, clear-bottom microplate. Supplier: Molecular Devices. The SpectraMax i3x reader provides theAbsorption was measured at 490 nm by utilizing a Spectra max i3x Multimode microplate reader (Molecular Devices). 2. Additionally, user-installable options allow the SpectraMax i3 System to grow with your changing application needs to fulfill and go beyond the standard reader applications, protecting your initial investment. The information below applies to a standard 96 well plate, set to a linear shake mode. 4 mg mL −1; 1. The MiniMax cytometer with SoftMax® Pro Software can be used to image and analyze a variety of cells. Detection of pro-inflammatory factors. KEY FEATURES • Combine cellular imaging and microplate assays into a workflow • Eliminate cell staining for cell counting and confluency with StainFree Technology The SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. Native or oxidized AN (40 μM) and FN (0. User-upgradeable detection modules allow you to expand the capabilities of your microp. Fluorescence Reader. The reader can be equipped with a NanoBRET detection for a 4-fold improvement in limit of. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader This page contains the table of contents of resources available for the SpectraMax Microplate Reader Suite of Products. SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax ® 340PC incorporates a holographic grating monochromator which allows you to specify a precise wavelength, from 340 nm to 850 nm, for the absorbance maximum of your sample. ZERO BIAS - scores, article reviews, protocol conditions and moreSpectraMax i3x offers all the techniques you need to explore cell pathway in a single system. The chemical reduction of resazurin was evaluated incubating each well of the microtiter plate with 10 μL (10% of final well volume) of 0. Singlet oxygen (1 O 2) generation was determined through 1,3-diphenylisobenzofuran (DPBF) quenching. Afterwards, 25 μL SpectraMax Glo Steady-Luc working solution was added to the wells containing luciferase and controls. SpectraMax Quant dsDNA Assay Kits. The Relative Luminescence Units (RLU) were measured using the SpectraMax i3x Luminescence Glow cartridge (Lum 384) (0200-7015POS, Molecular Devices) and normalized to the cell count or area, which were determined using the Mini MaxTM 300 Imaging Cytometer (5024062, Molecular Devices). Molecular Devicesの全商品を見る. The SpectraMax® i3x Multi-Mode Microplate Reader with the SpectraMax® MiniMax™ 300 Imaging Cytometer is equipped with transmitted light (TL), green fluorescence (Ex/Em: 460/541), and red (625/713) fluorescence channels. Spectral Fusion Illumination is a powerful combination of Xenon flash lamp and LEDs that provides unmatched signal strengthTNF-alpha quantification down to low picomolar levels on the SpectraMax Paradigm Platform. ZERO BIAS - scores, article reviews, protocol conditions and moreWe would like to show you a description here but the site won’t allow us. These robust readers have been placed in labs from Antarctica to the. techsupport. We would like to show you a description here but the site won’t allow us. BRET2canbeusedtostudyvariouskindsofprotein-proteininteractions. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Afterwards, 25 μL SpectraMax Glo Steady-Luc working solution was added to the wells containing luciferase and controls. The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. Explore a wealth of applications in one future-ready system that measures spectral-based absorbance, fluorescence, and luminescence. Equipped with transmitted light (TL), green (Ex/Em: 460/541) and red (625/713) fluorescence channels, the MiniMax cytometer with SoftMax® Pro Software can be used to image and. Description 1. Settings not required by a reader are indicated by ‘---’. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. The fluorescence quantum yield (QY) of the Pdots was measured by a UV−NIR absolute PL QY. Sunnyvale, CA, USA) with excitation at 475 nm. Using the SpectraMax i3x reader, the preconfigured SpectraMax Glo Steady-Luc Reporter assay protocol in SoftMax Pro Software was used to measure luminescence. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,*On the SpectraMax i3x reader only. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Then, the cell area was calculated for each. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. Check software compatibility with Windows 10 and locate your software installer. With a preconfigured protocol to simplify data acquisition and analysis, SoftMax Pro Software completes this total solution for fast, sensitive, and reliable dual luciferase reporter assay results. GFP expression was higher at 21 hours post transfection than at 16 hours (16-hour data not shown). SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. SoftMax Pro GxP Compliance Software. SpectraMax i3Instrumenttovisuallyinspectyoursampleandtoruncell-basedassaysat cellularorwhole-cellresolution. 1. The Molecular Devices SpectraMax i3X Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence. We would like to show you a description here but the site won’t allow us. Category 2 and 3 microplate readers use gradient heating as well. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. For more detailed information and technical support of Life Technologies assays, please call 1-800-955-6288 and enter extension 40266 or email drugdiscoverytech@lifetech. Actual shake speed is based on the microplate format. KEY FEATURES • Combine cellular imaging and microplate assays into a workflow • Eliminate cell staining for cell counting and confluency with StainFree TechnologyWe would like to show you a description here but the site won’t allow us. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. 2 in. SpectraMax Microplate Readers. 1. A SpectraMax® i3x was used to determine fluorescence with an excitation wavelength of λ = 360 nm and emission at λ = 460 nm. Molecular Devices社の製品: 吸収度マルチモードマイクロプレートリーダー SpectraMax® i3x に関するすべての情報をご覧ください。. SpectraMax i3x读板机针对96孔板和384孔板进行检测时，最低检测下限可至10细胞每孔。 此种检测方法的灵敏度远远高于标准比色法和绝大多数荧光法。 CellTiter-Glo试剂检测线性范围覆盖所有细胞浓度区间，动态学检测范围大于3个数量级且r2 > 0. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry leading SoftMax Pro. Additionally, user-installable options allow the SpectraMax i3 System to grow with your changing application needs to fulfill and go beyond the. Contact Local Team. Spectramax I3x, supplied by Danaher Inc, used in various techniques. the SpectraMax Paradigm Multi-Mode Detection Platform User Guide. Benefits • User-upgradeable application modules including cellular imaging • Sensitivity across spectrum with Spectral Fusion™ Illumination • Expanded dynamic range with cooled PMT • Control and analytics provided by SoftMax® Pro Software SpectraMax i3x The SpectraMax i3x from Molecular Devices is a multi-mode detection system that evolves with your future needs and offers an. Published Dec 16, 2020 | Updated Dec 16, 2020. 吸収度マイクロプレートリーダー SpectraMax® Mini. , 2021). SpectraMax® iD3/iD5. Multimode Reader Comparison Chart. The intensity of the emitted light can be measured easily with the SpectraMax i3x reader. Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. Additionally, user-installable options allow the SpectraMax i3 System to grow with your changing application needs to fulfill and go beyond the standard reader applications, protecting your initial investment. The device can be upgraded to include capabilities for Western blotting, imaging and injection. Molecular Devices Software. 0001 0. 635. When used in combination with the SpectraMax Injector Cartridge, this system enables a wealth of flash type luminescence applications including reporter gene and other assays with exceptional sensitivity and dynamic range. ATP LLD (pM) ATP LLD (moles) SpectraMax i3x, luminescence . 13. 0´ 104 6. Prepare your i3/i3x instrument for shipment. 2. . The SpectraMax® i3x multi-mode microplate reader is a configurable, versatile microplate reader that evolves with your future needs and allows you to investigate every aspect of a cellular pathway. SpectraMax i3, i3x, and iD5 readers. 08, 0. The SpectraMax® i3x offers a wealth of applications; it can measure fluorescence, absorbance and luminescence, and its modular upgrades enable western blotting, imaging cytometry and injectors. SpectraMax i3x The SpectraMax i3x from Molecular Devices is a multi-mode detection system that evolves with your future needs and offers an unlimited breadth of application possibilities. pathways and protein activation and expression in one system.